tion in biomass ? Limitation of plant production by nitrogen ? Low resveratrol, resveratrol derivatives and emodin production. The efficiency of nitrogen fixation was significantly correlated using the ratio of resveratrol to resveratrol glucoside. This indicates that knotweed CAL-101 contributed to the energy price of nitrogen fixation for melilot and that there is an exchange of organic substances between these two plant species. There appeared to be differences between the substrates. Compost was revealed to have a low efficiency of N fixation and, at the same time, showed a greater proportion of resveratrol glucosides compared with its aglycones. The opposite was accurate for the clayish low nutrient substrates, clay and loess.
Clay of miocene origin was obtained from spoil banks that had been made up of the same material CAL-101 as the soil within the field experiment , loess from nearby loess deposits and compost was that employed for dump reclamation. The chemical composition of the substrates is shown in Table 2. Ten pots had been filled with 7.25 kg of clay every and 2 l of certainly one of the following substrates: loess ; compost , composed of a 1:1 mixture of common compost plus a cellulose rich paper mill by item called Lignocel ; or clay enriched with a slowrelease biofertilizer Conavit? ; or clay enriched with Conavit and 50 ml of arbuscularmycorrhizal item Symbivit? . For technical sheet and composition of both items see http: www. symbiom.cz. A mixture of six mycorrhizal fungi species with at least 80,000 living propagules per litre in zeolit or spongilit was added to every pot, in addition to expanded clay enriched with all-natural fertilizer.
Conavit is a entirely all-natural slow nutrient releasing fertilizer composed of sea algae, humus substances, ground minerals and rocks, and is a all-natural source of keratin. A quantity of Conavit corresponding Gefitinib to 160 kg ha was applied. Symbivit was added to the Conavit treated pots on prime of the bottom clay layer. The bottom layer of clay had a texture of larger lumps, whilst the overlying material was broken up into smaller particles. Twenty pots of every variant had been prepared for a total of 100 pots. The pots had been thoroughly wetted and kept within the greenhouse at 18 27 C. During the summer, the whole set was transferred outdoors to the experimental garden and was kept moist making use of automatic drop irrigation as important.
Plants At the start out of the experiment, November VEGF 18, 2005, segments of R. bohemica rhizomes that had been pre cultivated in peat had been cautiously prepared. Each pot received a segment of washed rhizome with a recognized fresh weight plus a recognized quantity of buds. The average fresh weight of a segment was 3.3 g and also the average bud number was 1.6. The bud numbers did not differ significantly between the variants. Around 40 extra segments of these rhizomes had been every inserted into a small pot of perlite as a way to create plantlets in case a number of the plants within the experimental pots failed to grow. This proved to be a great advantage because a number of the rhizomes, specially those from the variant grown with Conavit, did not create any plantlets. This is almost certainly on account of the adverse effect of humic substances on the growth of fine roots.
The dormant rhizomes had been later exchanged for mature plantlets from the perlite pots. The pre grown plantlets continued their growth devoid of restriction, regardless of which variety of substrate they had been transplanted into. Right after three months, the R. bohemica plants had been well established and white melilot seeds Gefitinib had been added to 10 out of the 20 pots of every variant. The ability of the seeds to germinate was assessed prior to seeding and was found to be 57 according to the average from 10 Petri dishes, every with 25 seeds. You will discover around 500 seeds in one gram. Right after the first season, the plants had been harvested in September 2006. We measured CAL-101 twig numbers, lengths and dry masses of both Reynoutria and Mellilotus, and excised 100 mm segments of the new rhizomes, which formed alongside the pot wall, for chemical analyses.
The ramification of the branches was also taken into account; the lengths of all the principal branches Gefitinib rising from the soil, as well as the lengths of all of the side branches, had been measured and evaluated. Fine roots had been sampled, whilst knotweed roots had been hand separated from the melilot roots, and both had been stained and inspected for the presence of mycorrhiza. The experiment was terminated immediately after the second season in September 2007. At the end of the experiment, both the aboveground and belowground biomass had been measured, the fine roots had been sampled for mycorrhiza and larger roots and rhizomes had been thoroughly washed making use of air and water pressure. These had been then dried and ground for analysis. Melilot was allowed to grow devoid of restriction during the first season, but plants had been repeatedly cut during the second season to sustain a height of 30 cm. Field experiment The centre of the 1 ha experimental non irrigated field is at a location of 50 35’N, 13
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